RE: Storage of frozen sections
Here are my cryostat-details:
After cutting the cryostat slides we let them dry overnight under a fan.
There are stories here that longer drying will result into more crisper
staining of membranous antigens than short drying. I have never checked this.
Next morning the slides are packed face-to-face (with little carton strips
at the glass-ends) in Parafilm and put back into carton boxes the slides
came in (Star Frost, coated). Six of those carton boxes can be stored in a
plastic Roche storage box. The Roche boxes are placed in an aluminium tower
in the -80°C freezer. We don't use any desiccants. As far as I know a
temperature of -80°C guarantees a very dry environment!
After removal of slides from the freezer, we let them sit still packed
under a fan for at least 10-15 min before unwrapping. Acetone-fixation is
done right after unwrapping.
Chris van der Loos
Academic Medical Center
Dept. of Cardiovascular Pathology
NL-1105 AZ Amsterdam
Gayle Callis wrote:
>Date: 10 Dec 2002 12:00:34 -0600
>From: Gayle Callis
>Subject: RE: Storage of frozen sections
>Patsy and Chris,
>Could you elaborate on how these sections are stored, in slide boxes (what
>kind?) sealed, zip lock baggies, etc? With dessicant in containers??? How
>long do you air dry before storage, and how long do you let them air dry
>before opening a container to prevent water condensation?? and before
>This would be helpful and I know there is additional information in
>Histonet Archives - was discussed a few years ago -
<< Previous Message | Next Message >>