RE: Storage of frozen sections

From:Chris van der Loos

Here are my cryostat-details:
After cutting the cryostat slides we let them dry overnight under a fan. 
There are stories here that longer drying will result into more crisper 
staining of membranous antigens than short drying. I have never checked this.
Next morning the slides are packed face-to-face (with little carton strips 
at the glass-ends) in Parafilm and put back into carton boxes the slides 
came in (Star Frost, coated). Six of those carton boxes can be stored in a 
plastic Roche storage box. The Roche boxes are placed in an aluminium tower 
in the -80C freezer. We don't use any desiccants. As far as I know a 
temperature of -80C guarantees a very dry environment!
After removal of slides from the freezer, we let them sit still packed 
under a fan for at least 10-15 min before unwrapping. Acetone-fixation is 
done right after unwrapping.

Chris van der Loos
Academic Medical Center
Dept. of Cardiovascular Pathology
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands

Gayle Callis wrote:
 >Date: 10 Dec 2002 12:00:34 -0600
 >From: Gayle Callis 
 >Subject: RE: Storage of frozen sections
 >Patsy and Chris,
 >Could you elaborate on how these sections are stored, in slide boxes (what
 >kind?) sealed, zip lock baggies, etc? With dessicant in containers??? How
 >long do you air dry before storage, and how long do you let them air dry
 >before opening a container to prevent water condensation?? and before
 >actual fixation.
 >This would be helpful and I know there is additional information in
 >Histonet Archives - was discussed a few years ago -

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