RE: Storage of frozen sections

From:"Monson, Frederick C."

My [method] included zip-locks, but no desiccant.  I normally transferred
the box [sans bag]directly to cryostat chamber in whose depths I permitted
thermal equilibration.  Then they behaved, and were treated, as any other
frozen section.

My longest was a year for one experiment.  Couldn't see the difference
between sections or tissue [in OCT] stored at -70/-80.  But just for a few
CD Ab's at the time.  As the tissue was easier to store, I only did that for
sections of normals.  As the results of the experiment were negative [no
change/no difference but good staining], the results[?] were not published.


Fred Monson

-----Original Message-----
From: Gayle Callis []
Sent: Tuesday, December 10, 2002 12:37 PM
To: Patsy Ruegg;
Subject: RE: Storage of frozen sections

Patsy and Chris,

Could you elaborate on how these sections are stored, in slide boxes (what
kind?) sealed, zip lock baggies, etc? With dessicant in containers???  How
long do you air dry before storage, and how long do you let them air dry
before opening a container to prevent water condensation?? and before
actual fixation.

This would be helpful and I know there is additional information in
Histonet Archives - was discussed a few years ago -

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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