RE: Storage of frozen sections
Chris describes by experiences with storing frozen sections to the tee.
Once I started storing cut FS unfixed and took care to remove them from the
-70 without allowing water to form, I have not seen any loss of antigenicity
on any antibody including hormone receptors stored this way for up to 6
years. Stored fixed FS have not performed this well.
Patsy Ruegg, HT(ASCP)QIHC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
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----Original Message Follows----
From: Chris van der Loos
Subject: RE: Storage of frozen sections
Date: Tue, 10 Dec 2002 09:35:38 +0100
Perhaps the following story about cryostat section storage is helpful to
Some years ago we used to store our cryo's acetone-fixed at -20C.
Unfortunately, it appeared that for example CD4 was lost after 2-3 months of
storage; CD3 was lost after 4-6 months. For a while I believed that cryostat
sections could not be stored much longer than a couple of weeks. Then, a
rheumatology research group explained me that they store their very small
biopsies fully cut as cryostat sections unfixed at -80C. They claimed to
have no loss of antigens, after 1-2 years of storage.
We have successfully adopted this method for our coronary artery atherectomy
specimens. Our oldest cyrostat sections are stored unfixed at -80C for a
couple of years now and so far they are still working for a lot of antigens
(CD3, CD4, CD8, CD68, T-cell activation markers, actin, etc.).
Chris van der Loos
Dept. of Cardiovascular Pathology
Academic Medical Center H0-120
NL-1105 AZ Amsterdam
Dolf Segers wrote:
>Date: 9 Dec 2002 03:45:12 -0600
>Subject: Storage of frozen sections
>Just a simple question. How long can frozen unfixed sections be stored in
>-60 C freezer. Sections are mounted on superfrost plus slides. Does anyone
>have experience with storage of sections, or does anyone have some advice
>how to optimally store our material.
>Dept. Experimental Cardiology, Thoraxcenter
>Erasmus MC, Rotterdam
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