RE: Storage of frozen sections
Perhaps the following story about cryostat section storage is helpful to you:
Some years ago we used to store our cryo's acetone-fixed at -20C.
Unfortunately, it appeared that for example CD4 was lost after 2-3 months
of storage; CD3 was lost after 4-6 months. For a while I believed that
cryostat sections could not be stored much longer than a couple of weeks.
Then, a rheumatology research group explained me that they store their very
small biopsies fully cut as cryostat sections unfixed at -80C. They claimed
to have no loss of antigens, after 1-2 years of storage.
We have successfully adopted this method for our coronary artery
atherectomy specimens. Our oldest cyrostat sections are stored unfixed at
-80C for a couple of years now and so far they are still working for a lot
of antigens (CD3, CD4, CD8, CD68, T-cell activation markers, actin, etc.).
Chris van der Loos
Dept. of Cardiovascular Pathology
Academic Medical Center H0-120
NL-1105 AZ Amsterdam
Dolf Segers wrote:
>Date: 9 Dec 2002 03:45:12 -0600
>Subject: Storage of frozen sections
>Just a simple question. How long can frozen unfixed sections be stored in a
>-60 C freezer. Sections are mounted on superfrost plus slides. Does anyone
>have experience with storage of sections, or does anyone have some advice on
>how to optimally store our material.
>Dept. Experimental Cardiology, Thoraxcenter
>Erasmus MC, Rotterdam
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