RE: Silanising slides

From:Jenny Oblander

Here is the method from Dr.David Brigati.

3-aminopropyltriethoxysilane (APTS)........10mls.
Acetone-certified ACS grade................490 mls.
Deionized distilled water

1.Mix acetone and APTS, place in a glass staining dish.
2.Fill 3 staining dishes with deionized distilled water.
3.Immerse racks in 1st dish(APTS/acetone) for 2 minutes.
4.Drain slides and dip 5 times in each dish filled with deionized D.W.*
5.Drain slides,place in a 80 degree C oven allow to dry for 30 minutes.
6.Store slides in dust free box at room temperature.
* change water after 5 racks of slides.

-----Original Message-----
From: zenobia haffajee []
Sent: Sunday, December 01, 2002 4:55 PM
To: Rosalba Zumbo
Subject: Re: Silanising slides

Hi Rosalba

This is the method we use.

Protocol for sialinization of slides

  Mix 10ml silane within 500ml acetone (solution is stable for 8 hours - the
colour will change with time), Dip clean (acid washed), dry slides in silane
solution for 2 minutes.  Dry slides in fume hood. Wash slides with 2 changes
of water (distilled). Dry completely in an oven and cool.

Hope this helps

Zenobia Haffajee



----- Original Message -----
From: "Rosalba Zumbo" 
To: "Histonet" 
Sent: Monday, November 25, 2002 3:43 PM
Subject: Silanising slides

> Hi All,
> Does anyone please have a rliable method for silanising slides. Different
> methods I have come across have varying results in regard to silver
> staining.
> Thanks
> Rosalba Zumbo
> Histology Manager
> Department of Forensic Medicine
> 50 Parramatta Rd
> Glebe NSW 2037
> PH: 02 85847842
> FAX: 02 95664573

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