RE: Alcohol used for Tissue Processing in Histology

From:"Luck, Greg D."


We use Flex 100 (from Richard Allan).  It is a "cocktail" of 60% isopropanol
and 40% methanol.  It has good dehydration action and works well on runs of
mixed tissue sizes and types.  In addition the cost is very economical.
Only draw back I've encountered is you cannot recycle it using a columnar
distillation system as we use for reprocessing our xylene.  Thanks,  Greg

Greg Luck
Anatomic Pathology Supervisor
Deaconess Medical Center
Spokane, WA 99204

-----Original Message-----
From: Jill Songer []
Sent: Tuesday, November 26, 2002 10:14 AM
To: Strauss, Brie I.; 'HistoNet Server'
Subject: Re: Alcohol used for Tissue Processing in Histology

We used to use pure ethanol for processing, but found it to be too 
dehydrating, especially for small species such as mice. We now use reagent 
grade alcohols.

At 10:48 AM 11/26/2002 -0500, Strauss, Brie I. wrote:
>Does anyone use reagent grade alcohol during tissue processing?
>Does anyone use pure ethanol during tissue processing?
>Brie Strauss
>Safety Assessment
>Merck & Co., Inc.
>West Point, PA
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Jill Songer HT (ASCP)
Histopathology Lab
Virginia Maryland Regional College
of Veterinary Medicine
Veterinary Teaching Hospital
Blacksburg, VA 24061

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