MacNeals is a wonderful stain for undecalcified bone sections in PMMA,
either microtomed or ground sections. 

A word of caution here.  Make your own MacNeals staining solution,
commercial brands for undecalcified bone use are absolutely horrible, been
there, tried one, and wasted a lot of money!  Don't waste your time with
commercial solution. It is easy to make up. 

It is a stable solution, actually sat around lab for a few years and still
worked with great results.  Ostoid never looked better!!! 


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu