GFP in frozen murine brains
The cryostat should be warmed up a bit, try -15 degrees C, and around 10 microns in thickness, or more if you're expecting weak expression. We have had good luck with using formaldehyde fixed (from paraformaldehyde), sucrose cryopreserved specimens for GFP expression on frozen sections, though we have not done brain tissues.
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
From: Melissa Gonzalez [mailto:Melissa.Gonzalez@cellgenesys.com]
Sent: Thursday, December 05, 2002 5:44 PM
To: HistoNet Server
Subject: RE: Daily Digest
Does anyone have any suggestions for cutting frozen mouse brains to look for GFP expression?
(As in how thick and approximate temperature of cryostat?)
<< Previous Message | Next Message >>