GFP in frozen murine brains

From:"Johnson, Teri"

The cryostat should be warmed up a bit, try -15 degrees C, and around 10 microns in thickness, or more if you're expecting weak expression.  We have had good luck with using formaldehyde fixed (from paraformaldehyde), sucrose cryopreserved specimens for GFP expression on frozen sections, though we have not done brain tissues.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

-----Original Message-----
From: Melissa Gonzalez []
Sent: Thursday, December 05, 2002 5:44 PM
To: HistoNet Server
Subject: RE: Daily Digest

Does anyone have any suggestions for cutting frozen mouse brains to look for GFP expression?
(As in how thick and approximate temperature of cryostat?)

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