Fwd:nonalchohol dehydration?

From:John Baker

>Hello Histonetters,  This question is being asked by one of our 
>faculty.  Any suggestions are welcome. Thank you in advance and have 
>a great weekend! John
>subject:  dehydration without alcohol
>We are looking for a way to dehydrate small trabecular bone struts 
>(approx. 100-200 microns diameter, 2 mm long) without using 
>alcohols.  The trabecular bone struts are vitally stained with 
>various fluorescent dyes, and ethanol has, in the past, caused us to 
>lose the dyes.  The struts will be fixed in PFA, then need to be 
>dehydrated in order to allow embedding in PMMA.

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G160
Ann Arbor, MI 48109-0486
Main lab office phone:734-763-9674
Histology office:734-936-1635

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