FW: wierd artifact on tissue


We experienced a similar problem and it was identified by a pathologist as inadequate removal of wax during the staining process. This can be caused by the xylene not dissolving out the wax properly. Check the times on your staining machine. Alternatively the machine is not being changed often enough.
Rita Russell
-----Original Message-----
From: S1522@aol.com [mailto:S1522@aol.com]
Sent: 04 December 2002 23:03
To: histonet@pathology.swmed.edu
Subject: wierd artifact on tissue

Hello all. I could really use some help. Today, the pathologist called to say that she is seeing an artifact on the tissue. It looks like clusters of fine PCP organisms, only that they are not. Like someone took a very fine tip black marker and made circles around the cells. She said it is not on every slide. The slides she had me check were prostate needle bx's and an antrum bx. The only change in our routine has been that we are demonstrating a recycling system for alcohol and Xylene. I have not used the recycled product on the tissue processor or the H&E autostainer, but I have placed the recycled Xylene in our glass Coverslipper.

I appreciate any and all comments.
Thank you.
Allan Memorial

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