Alcian Blue stains for Barrett's disease

From:"Jennings-Siena, Debbie"

Hi everyone,
I was wondering if anyone could suggest the appropriate Alcian Blue (and pH) stain for Barrett's disease of the esophagus.  I have to admit that trying to decipher all of the mucins boggles my mind.  There is a discussion going on here at Baylor as to whether Alcian Blue pH 2.5 or pH 1.0 is better and is it better with or without PAS counterstain.  I know that some of you Histology Guru's will have the answer readily available.  Thanks in advance for any and all help.
Debbie J. Siena, HT(ASCP)QIHC
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465 vm
214-820-4110 fax

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