rat tendon & muscle

From:Cathy Mayton

Fellow Histonetters,

I am posting this for my sister-in-law who does not have a computer.  So here goes.........

She has a study processing rat tendon (supraspinatus) and muscle excised from the shoulder.  The muscle measures approximately 1.5 cm x 1.5 cm with the point of the tendon measuring 2 mm.  The tissue is paraffin embedded using Paraplast Plus. The blocks are being sectioned longitudinally.  The problem is that "chunks" of the tendon are falling out of the sections.  The tendon is the point of interest for the investigator.  She is asking for processing suggestions or protocols that may be working in your lab.

Please respond directly to Lois Mayton.  Her work number (Hutzel Hospital) is 313-966-0640 and her home phone is 734-246-4864.  She is located in Detroit, Michigan.

Thank you in advance,

GLP Compliant Laboratory

Cathy A. Mayton
Project Director
Wasatch Histo Consultants, Inc.

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