Hi,
Does anyone out there have an IHC procedure for staining human tissue with
human Mab's? I have tried to do this by blocking endogrnous peroxidase with
H2O2,  IgG activity with a FAB fragment, protein with normal serum, and
biotin with the vectorlabs biotin blocking sysytem, and still have so much
background and non-specific staining in the negative controls (IgG whole
molecule) that the slides are unreadable.
Any suggestions would be greatly appreciated.
Thanx,
steve