what i would do for this is use lots of normal human serum to block with before
the primary antibody and again before the secondary, i would also put human
serum in my secondary reagent.  by a lot i mean about 40% solution.
patsy ruegg

"Crochiere, Steve" wrote:

> Hi,
> Does anyone out there have an IHC procedure for staining human tissue with
> human Mab's? I have tried to do this by blocking endogrnous peroxidase with
> H2O2,  IgG activity with a FAB fragment, protein with normal serum, and
> biotin with the vectorlabs biotin blocking sysytem, and still have so much
> background and non-specific staining in the negative controls (IgG whole
> molecule) that the slides are unreadable.
> Any suggestions would be greatly appreciated.
> Thanx,
> steve