Re: Paraffin Survey Results
Trisha
We use 1-5% fabric softener for an hour or longer after decalcification of
bone and have no problems cutting bone. The commercial manufacturers of
fabric softeners claim that it is the surfactant properties that allow for
better penetration of a fabric by detergent. In the chapter on bone in
the "theory and practice of histological techniques" by Bancroft and
Stevens it recommends using either long processing times or double
embedding to render bone cuttable. This suggests that it isn't the
properties of the paraffin but enhanced paraffin infiltration that it is
critical in obtaining high quality sections from bone. If you have bone
that you has already been processed, taking it back to paraffin or even
back to absolute and leaving them in the latter part of the processing
cycle for extended periods of time should resolve the problem
Gerard Spoelstra
School of Biomedical and Veterinary Science
Medical Scientist
Murdoch University
Western Australia
At 04:08 PM 18/12/01 -0800, you wrote:
Would those who work with bone, please, tell me if you use a particular
brand for that?
I am convinced I need to find a better paraffin than I have been using.
Thanks,
Trisha Emry
U of Washington
Orthodontics Research Lab
On Tue, 18 Dec 2001, Scott Taft wrote:
> Histonet people,
>
> 1. The following number of labs (who responded to my
> survey) use this paraffin for infiltration:
>
> Paraplast Xtra-7
> Surgipath INF-6
> Tissue Prep2-6
> Paraplast-6
> Tissue Prep-5
> Paraplast Plus-3
> Richard Allen #6-2
> Richard Allen #9-1
> Polyfin from TBS-1
> Fisher Ribbon Pro-1
> Surgipath Blue Ribbon-1
> NSH Wax (?)-1
> BDH Gurr Paramat-1
> Tissue Tek III-1
> BDH Paramat Pastillated-1
> Surgipath Formula R-1
> Gulfwax-1
>
> 2. The following number of labs (who responded to my
> survey) use this paraffin for embedding:
>
> Paraplast Xtra-7
> Surgipath EMB -7
> Tissue Prep2-7
> Paraplast-6
> Tissue Prep-3
> Paraplast Plus-2
> Richard Allen #6-2
> Polyfin from TBS-1
> Richard Allen #9-1
> Fisher Ribbon Pro-1
> Surgipath Blue Ribbon-1
> NSH wax ???-1
> Vip-1
> BDH Gurr Paramat-1
> Tissue Tek III-1
> BDH Paramat Pastillated-1
> Surgipath Formula R-1
> Gulfwax-1
> Peel AWay-1
>
> If you haven't heard of any of the paraffins listed
> above, email our friends in the "land down-under". I
> would like to know what NSH is myself.
>
> Thanks if you participated,
> Scott Taft HT ASCP
> Tucson, AZ
>
>
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