Re: Bodians stain
From: | =?iso-8859-1?q?Tony=20Henwood?= |
Ray,
The following might help:
LOOT'S MODIFIED BODIAN STAIN FOR NERVOUS TISSUE
NOTES:
The protargol solution used for impregnation is
modified by the addition of hydrogen peroxide to delay
the darkening of the solution and copper nitrate to
improve selectivity.
SOLUTIONS:
1. Protargol Solution:
Dissolve 0.5g Protargol in 50 ml
distilled water,
Add with continuous agitation:
1% Copper Nitrate 1
ml.
1% Silver Nitrate 1
ml.
30% Hydrogen Peroxide 0.05
ml.
2. Reducing Solution I:
Hydroquinone 0.25
g.
Concentrated formalin 2.5
ml.
Distilled Water 50
ml.
3. 1 % aqueous Gold Chloride
4. Reducing Solution II:
Oxalic Acid 1 g.
Concentrated Formalin 2.5
ml.
Distilled Water 50
ml.
5. 5% Sodium Thiosulphate
STAINING PROCEDURE:
1. Dewax and hydrate sections to distilled water.
2. Place in Protargol Solution 37oC 48 hours.
3. Rinse slides briefly in distilled water.
4. Place in Reducing Solution I 5 minutes.
5. Rinse in water and check microscopically, if
underimpregnated return to Protargol solution for a
further 24 hours.
6. Tone in Gold Chloride 10 minutes.
7. Rinse in water.
8. Reduce in Reducing Solution II 10 minutes.
9. Rinse and fix slides in Sodium Thiosulphate 2
minutes.
10. Rinse in water, dehydrate, clear and mount.
RESULTS:
Neurfibrils, nerve fibers, axons Dark
red to black
Collagen
Light red or grey.
REFERENCE:
Loots, J.M., etal (1977) Stain Technol
52(2):85-87.
--- Ray Lopez wrote: > Help,One of
our Pathologist has just asked for
> bodians stain I have not done
> one in years ,does anyone out there have any
> information/procedures. Any
> help would be greatly appreciated.
>
=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
WESTMEAD, 2145
AUSTRALIA
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