Re: Bodian Stain
Dear HistoNetters
Happy Holidays!
Here's a nice microwave version of the Bodian Stain, courtesy of Dr.
Mathilde Boon:
The Bodian silver stain is used to color neurofibrils, unmyelinated and
myelinated nerve fibers.
SOLUTIONS NEEDED:
Protargol solution:
0.5 g Protargol
3.0 g Metallic Copper Shot
50 ml Distilled water
NOTE: Prepare the solution immediately before use.
Developer solution:
1 g Hydroquinone (C6H6O2)
50 ml Distilled water
2.5 ml 37% formalin (CH2O)
NOTE: Prepare the solution immediately before use.
Gold Chloride solution:
1 g Gold (III) Chloride (HAuCl4·H2O, 49% pure)
100 ml Distilled water
3 drops Glacial Acetic acid
Oxalic Acid solution:
1 g Oxalic acid dihydrate (C2H2O4·2H2O)
50 ml Distilled water
Sodium Thiosulfate solution:
5 g Sodium Thiosulfate (Na2S2O3·5H2O)
50 ml Distilled water
PROCEDURE:
1. Deparaffinize and bring to water
2. 60 min / 37°C in conventional incubation / 50 ml of Protargol solution
3. 60 min / 60°C / same Protargol solution
4. Rinse well in distilled water
5. 10 min / 50 ml Developer solution
6. Rinse well in distilled water
7. 2 min / 60°C / 16 ml Gold Chloride solution
8. Rinse well in distilled water
9. 10 min / 50 ml Oxalic Acid solution
10. Rinse well in distilled water
11. 10 min / 50 ml Sodium Thiosulfate solution
12. Rinse well in distilled water
13. Dehydrate and mount
RESULTS:
Neurofibrils, unmyelinated and myelinated nerve fibers black
Nucleoli black
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