Have you tried Dako's regular Target retieval Solution? That's what I use with their antibody at 1:10 too on FFPEhuman tissue, using a LN as a control.
>>> Elaine Dooley 12/20/01 01:59PM >>>
Has anyone had success staining formalin fixed paraffin embedded sections for BCL6. I have tried heat induced epitope retrieval with Trilogy (Cell Marque)and high ph target retrieval from DAKO on tonsil sections. I have tried both companies antibodies. The best staining I had was with high ph target retrieval and the antibody at 1:10. But I need to get stronger staining. I have tried amplification on a Ventana immunostainer and also tried staining by a manual method and it is just not dark enough.
Would appreciate any suggestions
Elaine Dooley HTL
352-265-0111 ext 4-4935
Thanks in advance
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