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Sigma has a kit for this (probably the easiest way to do this stain).  It's called Leukocyte naphthol AS-D Chloroacetate esterase, and the kit number is 91-C.  It can be done on paraffin sections, using appendix as a control.  Neutrophils stain a bright red.
 
Teri Johnson 
Manager Histology Core Facility 
Stowers Institute for Medical Research 
1000 E. 50th St. 
Kansas City, Missouri  64110 
tjj@stowers-institute.org 

-----Original Message-----
From: Keith Rogers [mailto:krogers@ncifcrf.gov]
Sent: Friday, December 28, 2001 7:11 AM
To: HistoNet Server
Subject: Leder Stain


Hello Everyone,

Does anyone have a method or a reference for "Leder Stain" for neutrophils?

Thanks in advance,

Happy New Year,

Keith 
Keith
http://web.ncifcrf.gov/rtp/labs/lasp/phl/default.   asp  



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Sigma 
has a kit for this (probably the easiest way to do this stain).  It's 
called Leukocyte naphthol AS-D Chloroacetate esterase, and the kit number is 
91-C.  It can be done on paraffin sections, using appendix as a 
control.  Neutrophils stain a bright red.
 

Teri Johnson 
Manager Histology Core Facility 
Stowers Institute for Medical Research 
1000 E. 50th St. 
Kansas City, 
Missouri  64110 
tjj@stowers-institute.org 

  
-----Original Message-----
From: Keith Rogers 
  [mailto:krogers@ncifcrf.gov]
Sent: Friday, December 28, 2001 7:11 
  AM
To: HistoNet Server
Subject: Leder 
  Stain


  
Hello Everyone,


  
Does anyone have a method or a reference for "Leder Stain" for 
  neutrophils?


  
Thanks in advance,


  
Happy New Year,

Keith 
Keith
http://web.ncifcrf.gov/rtp/labs/lasp/phl/default.asp


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