Keith,

This is one of several methods to demonstrate chloroacetate esterase activity.
The method of Leder utilizes hexazotized pararosaniline.
The reference is;

Leder LD. Uber die seleketive fermentcytochemische darstellung von neutrophilen myeloischen zellen und gevebsmastzellen im paraffinschmitt. Klin Wochenschr 42:553,1964.

The method( a modified version of the Fast Garnet GBC method of Moloney) may be found on pp 416-417, The Theory and Practice of Histological Techniques. Bancroft JD. and Stevens A., 4th Edition 1996, Churchill Livingstone, ISBN 0-443-04760-X.

regards

Bryan

----------
From:   Keith Rogers[SMTP:krogers@ncifcrf.gov]
Sent:   December 28, 2001 8:11 AM
To:     HistoNet Server
Subject:        Leder Stain

Hello Everyone,

Does anyone have a method or a reference for "Leder Stain" for neutrophils?

Thanks in advance,

Happy New Year,

Keith
Keith
http://web.ncifcrf.gov/rtp/labs/lasp/phl/default. asp

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