|From:||Hewlett Bryan |
This is one of several methods to demonstrate chloroacetate esterase activity.
The method of Leder utilizes hexazotized pararosaniline.
The reference is;
Leder LD. Uber die seleketive fermentcytochemische darstellung von neutrophilen myeloischen zellen und gevebsmastzellen im paraffinschmitt. Klin Wochenschr 42:553,1964.
The method( a modified version of the Fast Garnet GBC method of Moloney) may be found on pp 416-417, The Theory and Practice of Histological Techniques. Bancroft JD. and Stevens A., 4th Edition 1996, Churchill Livingstone, ISBN 0-443-04760-X.
From: Keith Rogers[SMTP:firstname.lastname@example.org]
Sent: December 28, 2001 8:11 AM
To: HistoNet Server
Subject: Leder Stain
Does anyone have a method or a reference for "Leder Stain" for neutrophils?
Thanks in advance,
Happy New Year,
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