RE: Clearing agents

From:"Johnson, Teri"

I've vowed to keep xylene out of my lab, so I'm using ProPar for processing and Clear-Rite 3 in my staining table. I have some toluene on hand in case I need to soak off coverslips and thin the mounting medium.  The only bone I've done is mouse tibia and femur, and it works quite well with these.

Teri Johnson
Manager Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj@stowers-institute.org


-----Original Message-----
From: DeLovino, Salvacion S. [mailto:Salvacion.DeLovino@cshs.org]
Sent: Friday, December 21, 2001 8:26 AM
To: HistoNet@pathology.swmed.edu; 'P. Emry'
Subject: RE: Clearing agents


chloroform for breast and fatty tissues and xylene for all others

Salvacion Delovino
> ----------
> From: 	P. Emry[SMTP:emry@u.washington.edu]
> Sent: 	Thursday, December 20, 2001 7:13 PM
> To: 	HistoNet@pathology.swmed.edu
> Subject: 	Clearing agents
> 
> Now that we have had a great review of paraffins, could I ask what
> clearing agents ya'all are using..specially for bone?
> 
> Thanks,
> 
> Trisha
> 
> 
> 





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