RE: BCL6
Elaine,
Try: DAKO Bcl-6 @ a 1:40 dilution. Control: Tonsil & lymphoma. Retrieve in
DAKO pH 10.0 target retrieval solution @ 100 degrees centigrade for 35
minutes followed by a 20 min. room temp. cool down. Apply antibody for
45-60 min. and use LSAB+ detection with DAB staining. Remember to use
silanized slides to avoid floating. This works very well for me. If you
want dark staining, go 1:25 on the dilution.
Good Luck,
Glen A. Dawson BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI
-----Original Message-----
From: Elaine Dooley [mailto:DOOLEEO@shands.ufl.edu]
Sent: Thursday, December 20, 2001 12:59 PM
To: Histonet@pathology.swmed.edu
Subject: BCL6
Dear Histonetters,
Has anyone had success staining formalin fixed paraffin embedded sections
for BCL6. I have tried heat induced epitope retrieval with Trilogy (Cell
Marque)and high ph target retrieval from DAKO on tonsil sections. I have
tried both companies antibodies. The best staining I had was with high ph
target retrieval and the antibody at 1:10. But I need to get stronger
staining. I have tried amplification on a Ventana immunostainer and also
tried staining by a manual method and it is just not dark enough.
Would appreciate any suggestions
Elaine Dooley HTL
Gainesville Fl
352-265-0111 ext 4-4935
Thanks in advance
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