From:"Dawson, Glen"


Try: DAKO Bcl-6 @ a 1:40 dilution.  Control: Tonsil & lymphoma.  Retrieve in
DAKO pH 10.0 target retrieval solution @ 100 degrees centigrade for 35
minutes followed by a 20 min. room temp. cool down.  Apply antibody for
45-60 min. and use LSAB+ detection with DAB staining.  Remember to use
silanized slides to avoid floating.  This works very well for me.  If you
want dark staining, go 1:25 on the dilution.

Good Luck,

Glen A. Dawson  BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI

-----Original Message-----
From: Elaine Dooley []
Sent: Thursday, December 20, 2001 12:59 PM
Subject: BCL6

Dear Histonetters,

Has anyone had success staining formalin fixed paraffin embedded sections
for BCL6.  I have tried heat induced epitope retrieval with Trilogy (Cell
Marque)and high ph target retrieval  from DAKO on tonsil sections.  I have
tried both companies antibodies.  The best staining I had was with high ph
target retrieval and the antibody at 1:10.  But I need to get stronger
staining.  I have tried amplification on a Ventana immunostainer and also
tried staining by a manual method and it is just not dark enough.

Would appreciate any suggestions

Elaine Dooley HTL
Gainesville Fl 

352-265-0111 ext 4-4935

Thanks in advance

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