Su, Phy-Huynh -
  There are many methods available for musculoskeletal tissues. There are no
set guidelines or references. We all seem to develop a system that works for
our particular area. The histonet archives may be helpful.  A literature
search too.  The book, "Methods of Calcified Tissue Preparation", Dickson,
Elsevier Press:1984, is broad based and very helpful.  I am involved with a
hard tissue group, feel free to contact me.  Many people working in bone, do
not read this forum regularly.  Good Luck!
Vicki Kalscheur, HT (ASCP) MLT
Comparative Orthopaedic Research Laboratory
University of Wisconsin-Madison
2015 Linden Drive West
Madison, WI 53706-1102     ph  608-262-8534