I believe it is spelled sirius red, and is commonly used as a stain for
amyloid, the Sweat-Puchtler method.  AFIP manual has a protocol, the
reagent recipes you use for bone could very well be the same as this
method.  Might help you out.


Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)