immuno histo chemistry
To all Histonetters,
I have a problem with my staining and need help.
Recently I am having problems with back ground staining.
Until 2 days ago every thing looked fine,I did a PM on the auto immuno
stainer and thats when the problem started.
The procedure is the same from start to finish but what I find now is a lot
of back ground staining. There is faulse positive staining and where a
cytoplasmic staining or membrane staining is required the nuclear also
stains It is very frustrating.
If anybody could shed some light on this problem I will appreciate it.
Thanks in advance.
Mountain View Ca.
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