formalin fixation of tissues for immuno's, research

From:Kathleen Cormier

Hello All,

I was wondering, I am curently getting back into immuno's in a research
setting, ack! It's been a long time, and my brain is long is
too long for formalin fixation for immuno's. The general rule of thumb here
is 24 hrs best, no more than 36 hours. Has anyone else noticed this
excessive sensitivity  to formalin? We do do antigen retrival, and some
digestion techniques on all the slides. Is this just the nature of the
beast? Has anyone found a nice easy to use all around fixative that works
well with immuno and that does not render the tissue hard as a rock to
section? Thanks all!

Histo Supervisor
Division of Comparative Med

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