Re: temperatures and immunostaining
Dear Histonetters,
In 1997, I participated in a project at Deborah Research Institute on just
this subject. We used a microwave outfitted with a fiberoptic probe and
varied the temperatures of the immunostaining reactions by just a degree at
a time over a small range of temperatures. The effects were quite dramatic.
The results were published in Cell Vision magazine and in:
Choi T-S, Whittlesey MM, Slap SE (1997) Microwave immunohistochemistry:
advances in temperature control. In: Gu, J. Analytical Morphology: Theory
Applications and Protocols, Boston, Eaton Publishing, 91-114
best regards,
Steven Slap
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Marketing Manager/Microwave Product Specialist
Hacker Instruments & Industries, Inc.
http://www.hackerinstruments.com
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