sounds like your sections may have dried out over night.
patsy ruegg

FARFAT@aol.com wrote:

> To all Histonetters,
> I have a problem with my staining and need help.
> Recently I  am having problems with  back ground staining.
> Until 2 days ago every thing  looked fine,I did  a PM on  the auto immuno
> stainer  and thats when the problem started.
> The procedure is the same from start to finish but  what I find now is a lot
> of  back ground  staining. There is faulse positive staining and where  a
> cytoplasmic staining or membrane staining is  required   the nuclear  also
> stains It is very frustrating.
> If anybody could shed some light  on this problem  I will appreciate it.
> Thanks in advance.
> Akbar.
> Mountain View Ca.