Re: immuno histo chemistry
sounds like your sections may have dried out over night.
> To all Histonetters,
> I have a problem with my staining and need help.
> Recently I am having problems with back ground staining.
> Until 2 days ago every thing looked fine,I did a PM on the auto immuno
> stainer and thats when the problem started.
> The procedure is the same from start to finish but what I find now is a lot
> of back ground staining. There is faulse positive staining and where a
> cytoplasmic staining or membrane staining is required the nuclear also
> stains It is very frustrating.
> If anybody could shed some light on this problem I will appreciate it.
> Thanks in advance.
> Mountain View Ca.
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