Hi everyone,

As some of you may know that I post a questions regarding CD31 and 
pretreated the slides with DAKO Target Retrieval Solution. However, my 
tissue got eaten up and the mophology look back. Some tissues even about to 
come off the slides. I try to be very careful when I take the slides out and 
washing the slides.

I did as followed:
1) Deparaffinized (Histoclear to water)
2) Washed slides in TBST 3x for 5 min.
3) Pretreatment with TRS


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