Re: cork for frozen samples

From:rkline@emscience.com

Barry,

I used cork stoppers for years for freezing and storing muscle biopsies in
isopentane and never experienced any of the problems I am now reading about.

Rande Kline
EM Science




Barry Rittman  on 12/12/2001 12:12:31 AM

To:   histonet@pathology.swmed.edu
cc:    (bcc: Rande Kline/EMI/Merck)
Subject:  Re: cork for frozen samples



Rande
Must disagree with you. Most cork stoppers have air pockets in them and if
slices from them are used they will tend to explode after stored samples on
them are taken out of liquid nitrogen. We still have some walls decorated
with such samples.
We found that it was best to go to the local hardware store and but best
quality cork sheeting, the compressed type. Then cut circles using cork
borer.  For us this has proved to be significantly cheaper than purchasing
from a company.
Barry


At 04:19 PM 12/11/2001 -0500, you wrote:
>Hazel,
>
>Try slicing cork stoppers.
>
>Rande Kline
>EM Science
>
>
>
>
>Hazel Stevens  on 12/11/2001 02:47:50 PM
>
>To:   "Histonet@pathology.swmed.edu" 
>cc:    (bcc: Rande Kline/EMI/Merck)
>Subject:  cork for frozen samples
>
>
>
>
>Hi,
>
>Does anyone know of a company that sells cork discs/board for freezing
>small specimens. I used to know of some in the UK but now I've moved out
>to California  it's not so easy knowing who to contact.
>
>Many thanks
>
>Hazel Stevens
>
>
>
>Hazel Stevens,
>Tissue Engineering Science Laboratory
>6405 EBU1
>Department of Bioengineering
>University of California, San Diego,
>9500 Gilman Drive,
>La Jolla,
>CA 92093-0412,USA
>
>Tel: (858) 534 1765
>Fax: (858) 822 0240
>
>
>
>
>
>
>
>
>









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