Re: Seawater Composition URL's

From:"Monson, Frederick C."

To those who are interested in URL's at which info about sea water
composition is available, I forward this.

> ----------
> From: 	Monson, Frederick C.
> Sent: 	Friday, December 7, 2001 9:31 AM
> To: 	'Dr. Ian Montgomery'
> Cc: 	HistoNet Server (E-mail)
> Subject: 	RE: Fixation buffer.
> 
> Morning (here!) Ian,
> 	I have been looking as well, and haven't found anything to respond
> to your question, but, as usual, I have found a goldmine website for
> seawater data, so I thought I would share it for those who might find a
> use
> for artificial seawater compositions:
> http://hermes.mbl.edu/BiologicalBulletin/index.html.  Another with a
> PDF-publication is:
> http://www.google.com/search?q=saltwater+fish+fixative&hl=en&start=10&sa=N
> .
> 
> Hope it's not too late.
> 
> Fred Monson
> 
> Frederick C. Monson, PhD 
> Center for Advanced Scientific Imaging(CASI) 
> West Chester University of Pennsylvania 
> Schmucker Science Center II  
> South Church Street                                                    
> West Chester, PA, 19383
> eMail:  fmonson@wcupa.edu
> http://darwin.wcupa.edu/casi/
> 
> 
> 
> > ----------
> > From: 	Dr. Ian Montgomery
> > Sent: 	Monday, December 3, 2001 12:01 PM
> > To: 	histonet@pathology.swmed.edu
> > Subject: 	Fixation buffer.
> > 
> >         Preparing fish larvae (sea) for EM, any suggestions for a
> suitable
> > buffering system given that the levels of salt in sea fish are  ~3 times
> > that of mammals. Do I simply make up the fixatives using sea water?
> > Sorenson buffered fixatives have a high osmolarity that might be another
> > buffer. Time, unfortunately, is short so I can't run a series of trials
> > although I would prefer that. 
> >         Back to mammals, lymphocytes and macrophage in mice, any
> > suggestions. I was going to look for esterases as a marker for
> macrophage
> > but I'm sure there is something better.
> > Ian. 
> > 
> > 
> > Dr. Ian Montgomery,
> > Microscopy Service Unit,
> > Graham Kerr Building,
> > Institute of Biological & Life Sciences,
> > University of Glasgow,
> > Glasgow,
> > G12 8QQ.
> > Tel: 0141 332 8855 Extn.6644.
> > e-mail: ian.montgomery@bio.gla.ac.uk
> > 
> 
> 




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