I use premade proteinase K from DAKO for usually 5 min.
Patsy

"Philopena, Jennifer" wrote:

> Hello.
> I'm looking for a mechanistic definition of Pro K and protease.
> I'm testing both as pre-treatments for hexon IHC.
> I've been using the Qiagen protease and it works well at 1mg/ml in PBS for
> 10 minutes, but my colleague got better staing than I did and I think she
> might have made a mistake in diluting the lyophilized powder.  I used to use
> Pro K, ran out, and grabbed what I thought was the next best thing, which
> was the Qiagen protease.
> If any of you use either digestion could you please send me specific
> information on the product - vendor and concentration.
> Thank you.  Happy holidays!
> Jen
>
> ***************************************************************
>  This message and any attachments is solely for the intended recipient. If
> you are not the intended recipient, disclosure, copying, use, or
> distribution of the information included in this message is prohibited --
> please immediately and permanently delete this message.