Wow!! Sorry about the typos

 --- Tony Henwood  wrote:
> Dolors,
> I searched my old lab manuals and found this:
> 
>                      MULLIGANS METHOD FOR MYELIN  
> (THOMPSON)
> 
> Principle:
> 
> Not clear but myelinated areas of the brain are
> stained blue.
> 
> Solutions:
> 
> A.   Mulligans solution:
>      Phenol Crystals                    5 g
>      Copper sulphate                    0.5 g
>      Hydrochloric Acid                  0.125 ml
>      Distilled Water                    100 ml
> 
> B.   2% Aqueous Ferric Chloride
> 
> C.   1% Potassium Ferrocyanide
> 
> D.   Acidic Formalin:
>      Formalin                           10 ml
>      Citric Acid                        0.2 g
>      Distilled Water                    90 ml
> 
> E.   Mounting Fluid:
>      Glycerine                          25 ml
>      Distilled Water                    75 ml
>      Formaldehyde                       10 ml
>      Citric Acid                        0.2 g
> 
> Method:
> 
> 1.   Ensure that brain slices are well fixed in 10%
> formalin.
> 
> 2.   Wash in running water, 1 hour.
> 
> 3.   Place for 5 minutes in solution A at 60oC.
> 
> 4.   Transfer to cold water, 10 seconds.
> 
> 5.   Transfer to solution B, 1 minute.
> 
> 6.   Wash in running water, 2 minutes.
> 
> 7.   Transfer to solution C, 4 minutes.
> 
> 8.   Wash in running water overnight.
> 
> 9.   Store in solution D for 2 months.
> 
> 10.  Mount in solution E.
> 
> Note:     These times are precise, especially in
> solution A.  Otherwise, white matter will take up
> the
> stain.
> 
> Reference:
> 
> Edwards, J.J. & M.J., "Medical Museum Technology". 
> Oxford Uni. Press, London, p. 87-88.
> 
> 
>  
> 
> --- Dolors Fuster  wrote: >
> Hi histonetters !
> > 
> > Does anybody have a protocol for Mulligan's stain?
> > 
> > I can't find it on the net
> > 
> > Thanks in advance :o)
> > Dolors Fuster
> > 
> > Tecnic Especialista en 
> > Anatomia Patologica
> > Departament d'Anatomia i Embriologia Humana
> > Facultat de Medicina (HCP)
> > Universitat de Barcelona
> > ICQ 14146798
> > 
> > Dolors Fuster
> > 
> > Tecnic Especialista en 
> > Anatomia Patologica
> > Departament d'Anatomia i Embriologia Humana
> > Facultat de Medicina (HCP)
> > Universitat de Barcelona
> > ICQ 14146798
> >  
> 


=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
WESTMEAD, 2145
AUSTRALIA

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