Dolors,
I searched my old lab manuals and found this:

                     MULLIGANS METHOD FOR MUELIN  
(THOMPSON)

Principle:

Not clear but myelinited areas of the brain are
stained blue.

Solutions:

A.   Mulligans solution:
     Phenol Crystals                    5 g
     Copper sulphate                    0.5 g
     Hydrochloric Acid                  0.125 ml
     Distilled Water                    100 ml

B.   2% Aqueous Ferric Chloride

C.   1% Potassium Ferrocyanide

D.   Acidic Formalin:
     Formalin                           10 ml
     Citric Acid                        0.2 g
     Distilled Water                    90 ml

E.   Mounting Fluid:
     Glycerine                          25 ml
     Distilled Water                    75 ml
     Formaldehyde                       10 ml
     Citric Acid                        0.2 g

Method:

1.   Ensure that brain slices are well fixed in 10%
formalin.

2.   Wash in running water, 1 hour.

3.   Place for 5 minutes in solution A at 60oC.

4.   Transfer to cold water, 10 seconds.

5.   Transfer to solution B, 1 minute.

6.   Wash in running water, 2 minutes.

7.   Transfer to solution C, 4 minutes.

8.   Wash in running water overnight.

9.   Store in solution D for 2 months.

10.  Mount in solution E.

Note:     These times are precise, especially in
solution A.  Otherwise, white matter will take up the
stain.

Reference:

Edwards, J.J. & M.J., "Medical Museum Technology". 
Oxford Uni. Press, London, p. 87-88.


 

--- Dolors Fuster  wrote: >
Hi histonetters !
> 
> Does anybody have a protocol for Mulligan's stain?
> 
> I can't find it on the net
> 
> Thanks in advance :o)
> Dolors Fuster
> 
> Tecnic Especialista en 
> Anatomia Patologica
> Departament d'Anatomia i Embriologia Humana
> Facultat de Medicina (HCP)
> Universitat de Barcelona
> ICQ 14146798
> 
> Dolors Fuster
> 
> Tecnic Especialista en 
> Anatomia Patologica
> Departament d'Anatomia i Embriologia Humana
> Facultat de Medicina (HCP)
> Universitat de Barcelona
> ICQ 14146798
>  

=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
WESTMEAD, 2145
AUSTRALIA

http://shopping.yahoo.com.au - Yahoo! Shopping
- Free CDs for thousands of Priority Shoppers!