Re: Mordant for PTAH

From:Ronnie Houston

My recollection of PTAH is that it would work well on routinely fixed paraffin
sections after a brief Mallory bleach (permanganate/oxalic). We also used it for the demonstration of gliosis, and usually stained both muscle and brain sections overnight at room temp. No need for any mordanting and beautiful results!
The trick was the differentiation in the alcohol  dehydration
The published protocol by the Botsford's seems too complicated and involved for my liking - exhausted after reading it - time for a Malt I think


Ronnie Houston
Cytochemistry & Molecular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
(214) 559 7744
(214) 559 7768 - fax

>>> Gayle Callis  12/07/01 10:12AM >>>
I always thought Zenkers (the mercury component being important, although
verbotten in labs these days) was used as the mordant for PTAH rather than
Bouins.  It is also my understanding, Zenkers postfixation "mordant" step
can be left out.  

Enlighten me.

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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