Re: Ki-67 on previous H&E

From:Amos Brooks

    If you have the sections on charged or sailinized slides, you should
have no problem. You actually needn't even destain them if you have plans to
use a hematoxylin counterstain since the eosin will come out in the alcohol
and water rinses before you retrieve the slides, and removing the
hematoxylin would be redundant. :-)
Amos Brooks

----- Original Message -----

> Need to do a Ki-67 on human tissue previously stained with a routine H&E
> using Mayers and Eosin with acetic acid. My gut tells me this will work,
> and yes I am doing HIER.  I expect there could be some background, but is
> there any chemical or molecular situation I am not thinking about that
> prevents this from working?

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