Re: Ki-67 on previous H&E

From:Richard Cartun

No, we do IP stains on H&E-stained tissue sections all the time.  Do you put your sections for H&E on "charged" slides?

RWC

>>>  12/10/01 05:19PM >>>
Need to do a Ki-67 on human tissue previously stained with a routine H&E
using Mayers and Eosin with acetic acid. My gut tells me this will work,
and yes I am doing HIER.  I expect there could be some background, but is
there any chemical or molecular situation I am not thinking about that
prevents this from working?







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