Re: Fixation Classification
The first question that comes to mind is "Why do fixatives have to be
classified?" Do we routinely use such a great number that we have to put
them into categories?
A fixative solution may contain several fixative agents each of which may be
additive or non-additive. Probably 99% of tissues are fixed in formaldehyde,
glutaraldhyde, alcohol, mercuric chloride, picric acid, potassium
dichromate, osmium tetroxide, or some combination of the above 6 reagents.
Individually, the reagents may be classified according to any criteria we
choose to select (additive/non-additive, coagulant/non-coagulant,
gaseous/non-gaseous, colored/colorless, aldehyde/non-aldehyde, etc). But,
once the individual agents are combined, their individual classification is
meaningless. We could, however, adopt the same approach used by wine
tasters; this fixative is "a well balanced blend of aldehyde, with a touch
of a coagulant heavy metal, a fine denaturing alcohol, and subtle tones of
picric acid".
As to the sugestion of using the terms "soft and hard" fixatives based on
nuclear appearances, this leaves me totally confused! What on earth is a
"soft-fixed nucleus"?
Paul
>From: Tony Henwood
>To: Histonet
>Subject: Fixation Classification
>Date: Fri, 14 Dec 2001 09:17:36 +1100 (EST)
>
>Hi all,
>Barry Rittman in August this year contributed to
>histonet:
>
>"Fixatives used to be described as additive or non
>additive.
>Additive fixatives are those in which the fixing agent
> attaches to the
>protein molecule.
>Non additive fixatives on the other hand cause changes
>in the proteins
>that cause them to become changed, often used to be
>described as
>denaturation.
>e.g. formalin., picric acid are additive, ethanol is
>non additive.
>Because of the complexity of fixation and the great
>variety of agents
>used, the terms additive and non additive are not now
>commonly used."
>
>The question I have been pondering is "What is the
>preferred classification for fixatives that we should
>use?"
>Luna suggested the terms hard and soft fixatives,
>basing his classification on nuclear appearance.
>What does everyone else think?
>
>Regards and Happy Christmas and a good new year
>
>Tony
>
>
>
>
>=====
>Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
>Laboratory Manager
>Histopathology Department
>The Childrens Hospital at Westmead
>Locked Bag 4001
>WESTMEAD, 2145
>AUSTRALIA
>
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