Thanks for all of the responses I got.  I think I will just
order it from Sigma as Tim suggested!

Kim





Tim Plummer wrote:

> Kim,
>
> Denhardt's Solution is a mix of BSA, ficoll, and PVP
> used as a blocking solution in molecular biology and
> is found in many prehybridization solutions for ISH.
> I find ordering it from Sigma as 50X (D2532 or
> D9905)is the best way to obtain it.  Preparing it from
> scratch is a pain, but less expensive (as usual).
>
> Tim Plummer
>
> --- Kimberly L Merriam  wrote:
> > Does anyone know what this is and how to make it?
> >
> > Thanks in advance,
> > Kim Merriam
> > TKT
> > Cambridge, MA
> > > begin:vcard
> > n:Merriam;Kim
> > tel;home:508-943-0417
> > tel;work:617-503-0374
> > x-mozilla-html:FALSE
> > org:Transkaryotic Therapies;Animal Physiology
> > adr:;;195 Albany St;Cambridge;MA;02139;
> > version:2.1
> > email;internet:kmerriam@tktx.com
> > title:Research Associate III, Histology
> > x-mozilla-cpt:;3
> > fn:Kim Merriam
> > end:vcard
> >
>
> __________________________________________________
> Do You Yahoo!?
> Check out Yahoo! Shopping and Yahoo! Auctions for all of
> your unique holiday gifts! Buy at http://shopping.yahoo.com
> or bid at http://auctions.yahoo.com