Kim,

Denhardt's Solution is a mix of BSA, ficoll, and PVP
used as a blocking solution in molecular biology and
is found in many prehybridization solutions for ISH. 
I find ordering it from Sigma as 50X (D2532 or
D9905)is the best way to obtain it.  Preparing it from
scratch is a pain, but less expensive (as usual).

Tim Plummer


--- Kimberly L Merriam  wrote:
> Does anyone know what this is and how to make it?
> 
> Thanks in advance,
> Kim Merriam
> TKT
> Cambridge, MA
> > begin:vcard 
> n:Merriam;Kim
> tel;home:508-943-0417
> tel;work:617-503-0374
> x-mozilla-html:FALSE
> org:Transkaryotic Therapies;Animal Physiology
> adr:;;195 Albany St;Cambridge;MA;02139;
> version:2.1
> email;internet:kmerriam@tktx.com
> title:Research Associate III, Histology
> x-mozilla-cpt:;3
> fn:Kim Merriam
> end:vcard
> 


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