Re: Cryostat static

Bob and Histonet,
Yes, this does seem to work. I got this tip from Tom Johnson of GTI.

Subj: Re: Cryostat static
Date: 12/6/01 6:02:07 PM US Mountain Standard Time
Sent from the Internet (Details)

In a message dated 12/03/2001 12:33:09 PM US Mountain Standard Time, writes:

<< Our pathology group has a LEICA CM1850 cryostat, My question to the
is......Does anyone experience static electricity when trying to pick up
sections?  When we try to pick up the sections they will fly up to the slide
or off on their own.  We would like to know how to remedy this if there is
any. Thanks for your help
Amylin Johnson
Associates in Pathology
Wausau, WI



Static can be a problem from time to time.  Here's a hint that was originally
passed along to Histonet by Andi Grantham at the University of Arizona, Dept.
of Cell Biology and Anatomy.  I have heard from several customers that this
remedy works:

Place a small beaker of about 70% or 80% ethanol in the cryostat chamber,
somewhere near the back and out of the way.  Close the door to the cryostat
and let set for 10-15 minutes.  Then resume sectioning.

For some strange reason, the ethanol fumes seem to cut down on the static and
make the sections behave themselves.  I know this sounds bizarre, but
apparently it does indeed work.

If you or anyone else on Histonet try this, let us know the results.  I don't
know if the concentration of the ethanol makes a real big difference.  If
Andi reads this, perhaps she will respond and fill us in on the particulars. 
But I know several people who have used 70% ethanol with good luck.


Bob Chiovetti
GTI Microsystems
Leica Exclusive Regional Dealer
Southwestern U.S.A.

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