Hi Ray,
Here's a Churukian Schenk that I used with bodian's.

Fixative:  10% neutral buffered formalin
Technique:  Cut paraffin sections at 3 to 5 microns
Quality Control:  An argyrophil-positive carcinoid tumor is preferred, but a
section of small intestive can be used.

Staining Procedure:

1.  Deparaffinize and hydrate sections to acidified Water, ph 4.2.
2.  Place slides in 0.5% silver nitrate and heat in microwave on "warm" for
2.5 min.
3.  Rinse in 3 changes of warm distilled water.
4. Heat Bodian's developer in microwave on "warm" for 2.5 min.  Place slides
in developer for 3 mins.
5.  Rinse in 3 changes of warmed distilled water.
6.  wash gently in running tap water.
7.  Rinse in 3 changes of warmed distilled water.
8.  Heat same silver nitrate solution as in step 2, on "warm" for approx. 2
mins. Place slides in warmed silver solution for 5 min.
9.  Rinse in 3 changes of warmed distilled water.
10. Heat same Bodian's developer as in step 4 on "warm" for approx. 2 mins.
Place slikdes in warmed developer for 3 min.
11 Rinse in 3 changes of warmed distilled water.
12. Counterstain with 0.1% nuclear fast red for 3 min.
13. Rinse in distilled water.
14. Dehydrate, clear, and mount.

Results:
     Argyrophil cells.....................................black
     Argentaffin substances..........................black
     Nuclei.....................................................red
     Backround...................................yellow-brown

Solutions:

0.3% Citric Acid
    Citric acid.............................................0.3 gm
    distilled water.....................................100.0 ml

Acidified water
     Distilled water.....................................500.0ml
     Ph to 4.2 with 0.3% citric acid sol'n.

0.5% Silver Nitrate
    Silver Nitrate........................................0.5 gm
    Acidified water.....................................100.0 ml

Bodian's Developer
     Sodium Sulfite (anhydrous).....................5.0 gm
     Hydroquinone........................................1.0 gm
     Distilled water....................................100.0 ml

Nuclear Fast Red  (Kernechtrot)
     Nuclear Fast Red..................................0.1 gm
     Aluminum Sulfate..................................5.0 gm
     Distilled water.....................................100.0 ml
Dissolve aluminum sulfate in distilled water, then add nuclear fast red and
dissolve with aid of gentle heat.  Cool, filter and add a grain of thymol
 as a preservative.

Reference:
      Churukian, C.J., and Schenk, E.A..  A modification of Pascual's
argyrophil method.  Journal of Histotechnology.  1979: 2: 102.
      Prophet, E.B.,  Mills, B., Arrington, J.B., and Sobin, L H.  1992.
Laboratory Methods in Histotechnology.  (pp. 189-190).


Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
  ----- Original Message -----
  From: Ray Lopez
  To: histonet@pathology.swmed.edu
  Sent: Friday, December 14, 2001 10:07 AM
  Subject: Bodians stain


  Help,One of our Pathologist has just asked for bodians stain I have not
done one in years ,does anyone out there have any information/procedures.
Any help would be greatly appreciated.