RISH In Situ counterstain

From:"Georger, Mary"

Good Morning,
Has anyone had experience with counterstaining RISH slides with Harris
hematoxylin rather than Mayers? A long sad story but now I find that I have
masked signal and am wondering if it could be the Harris.  Any wonderful
suggestions? I am wondering if I can take the coverslips off without
compromising the emulsion and destain in dilute HCl.
Thank  you in advance for any pearls of wisdom! 

Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642

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