RE: tissue preservation

From:"Monson, Frederick C."

Morning Judy,
	When I was a graduate student in the '60's of the last century(!!),
I decided that I wanted to store fixed tissues for a while.  Somewhere(?) I
found the following recipe:  70ml 95EtOH(USP) + 10ml
glycerin(1,2,3-propanetriol, glycerine, glycerol) + 15ml HOH.  In those
ancient days, I used a 2 dram shell vial with a cork with 1ml of the above,
the stopper sealed with beeswax.  All are gone now, but they lasted for 15
years until some began to break down in my garage.  Guyer, M.F.(1936),
recommends equal parts of glycerin, 95EtOH and HOH for storage of fixed

* EtOH - ethanol (all USP, i.e., never denatured!)


Fred Monson

Frederick C. Monson, PhD 
Center for Advanced Scientific Imaging(CASI) 
West Chester University of Pennsylvania 
Schmucker Science Center II  
South Church Street                                                    
West Chester, PA, 19383


> ----------
> From: 	Judy Trogadis
> Sent: 	Sunday, December 9, 2001 10:25 AM
> To:
> Subject: 	tissue preservation
> Hello, Histonetters:
> I have whole lung tissue, from rat, obtained by perfusion with 4%
> paraformaldehyde, removed, and then further fixed in PFA [that's
> paraformaldehyde ;-) ]  for 3 days. The plan is to collect thick vibratome
> sections, so the tissue will never be embedded. I would like to keep part
> of the lung for sectioning in the future and to carry out
> immunocytochemistry. What solution should I keep them immersed in? Right
> now they are in PBS, at 4deg C  but I feel that some preservative should
> be added to prevent decomposition. However, keeping them in fixative may
> reduce their antigenicity.
> I would appreciate hearing any suggestions from this very knowledgable
> group.
> Thank you
> Judy
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 8Q
> 30 Bond St.
> Toronto, ON M5B 1W8
> Canada
> ph:  416-864-6060  x6337
> fax: 416-864-6043

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