RE: laser capture microdissection

From:"Dawson, Glen"


We are performing laser capture microdissection here at the Medical College
of Wisconsin using Arctus (sp.?) equipment.  Essentially, I have been doing
CD20 IHC on 5um sections so that the desired cells really stand out.  I skip
counterstaining and just dehydrate through to 100% ethanol and then air-dry.
Do not simply airdry without ethanol dehydration as this seems to make the
cells adhere abnormally strong to the slide.  After they are airdried, I
hand them over to the pathologist for him or his assistants to do the
microdissection.  According to him, it is working fairly well. 

Glen A Dawson  BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI

ps.  Avoid excessive drying before performing IHC on the slides as well.
Looks like the less you bake the slides before IHC, the better for

-----Original Message-----
From: Johnson, Teri []
Sent: Tuesday, December 11, 2001 10:12 AM
To: Histonet (E-mail)
Subject: RE: laser capture microdissection

Please reply to the histonet.  We're evaluating this technology and the more
information we have, the better.

Teri Johnson
Manager Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

-----Original Message-----
From: Robert Geske []
Sent: Tuesday, December 11, 2001 9:34 AM
Subject: laser capture microdissection

Can anyone using laser capture microdissection comment on their experiences
using this technique for RNA and DNA isolation.  Whose instrument are you


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