Hi Nick
I recently did something similar on a host of breast aspirates. I found that
it was important to make sure that the mounting media was completely
removed.  I usually let the slides sit in xylene ( if using permount etc...)
for a few days and change frequently.  Hope this helps!
luis

-----Original Message-----
From: Nick_Madary@hgsi.com [mailto:Nick_Madary@hgsi.com]
Sent: Monday, December 10, 2001 5:20 PM
To: HistoNet@pathology.swmed.edu
Subject: Ki-67 on previous H&E


Need to do a Ki-67 on human tissue previously stained with a routine H&E
using Mayers and Eosin with acetic acid. My gut tells me this will work,
and yes I am doing HIER.  I expect there could be some background, but is
there any chemical or molecular situation I am not thinking about that
prevents this from working?