RE: Her-2/neu

From:"Dawson, Glen"

I am currently doing FISH correlations on a number of cases done both here
and elsewhere in which the patient tissue was a 3+ on core biopsies but were
negative on subsequent big blocks using the DAKO HercepTest kit.  I am also
monitoring the results closely on all HercepTest cases that I perform.
Preliminary results are leading me to believe that the temperature of both
the Kit reagents as well as the HercepTest wash buffer are a much bigger
factor than I had believed.  There is a standing order here (Medical College
of Wisconsin & Froedtert Hospital) to no longer do this test on breast core
bx's until this problem is resolved.  I'll let you know what I find out.
Glen A. Dawson  BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI

-----Original Message-----
From: Cathy Thornton []
Sent: Tuesday, December 11, 2001 9:17 AM
To: histonet
Subject: Her-2/neu

Is anyone using anything other than Herceptest for clinical Her-2/neu
stains.  If so what antibody and detection and could you discuss scoring and
reporting the results??  Also has anyone done FISH correlations with IHC
results within your own laboratory?
Pat Zeitlow
Boyce and Bynum Pathology Laboratories
Columbia, Missouri

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