Cathy:

I have been trying for 2 years to set up Her-2 neu testing in-house using
the Dako polyclonal Ab. and my TechMate auto-stainer.  I have done
correlations with results from a reference lab using Herceptest, but not
with FISH results.  Because of the subjective nature of the grading, and
despite the guidelines that I have provided (both data and image), I have
been unsuccessful in achieving concurrence among all 5 of my pathologists. 

Good Luck!!

Diana G. Goodwin
Dept. of Pathology
Capital Health System
750 Brunswick Ave.
Trenton,  NJ   08638
phone: 609-394-6000 ext.2122
fax: 609-278-5435
e-mail:  dgoodwin@chsnj.org


> ----------
> From: 	Cathy Thornton[SMTP:cthornton@usiw.net]
> Sent: 	Tuesday, December 11, 2001 10:17 AM
> To: 	histonet
> Subject: 	Her-2/neu
>
> Is anyone using anything other than Herceptest for clinical Her-2/neu
> stains.  If so what antibody and detection and could you discuss scoring
> and reporting the results??  Also has anyone done FISH correlations with
> IHC results within your own laboratory?
>  
> Pat Zeitlow
> Boyce and Bynum Pathology Laboratories
> Columbia, Missouri
>

Capital Health System
Trenton, New Jersey