RE: Fixation Classification
I am very much tempted to agree with you, but I'll try
to be an optimist
--- "Monson, Frederick C." wrote:
> My Mother spent at least two years of my youth
> drumming into me the need to
> say nothing when something that wasn't nice emerged
> from the recesses of my
> tortured brain. My wife despises sarcasm, but for
> this question I must be
> forgetful and negligent of their well-conceived and
> proper teachings.
> Fixation Classification: As long as our collective
> knowledge of organic and
> biochemical pathways and mechanisms is so poor and
> pathologists, immunohistochemists, flow
> cytometrists, histotechnologists and
> journal editors believe that a fixative called, "4%
> paraformaldehyde" is
> equivalent to "4% formaldehyde", why worry about
> something like a meaningful
> Fred Monson
> Frederick C. Monson, PhD
> Center for Advanced Scientific Imaging(CASI)
> West Chester University of Pennsylvania
> Schmucker Science Center II
> South Church Street
> West Chester, PA, 19383
> eMail: email@example.com
> > ----------
> > From: Tony Henwood
> > Sent: Thursday, December 13, 2001 5:17 PM
> > To: Histonet
> > Subject: Fixation Classification
> > Hi all,
> > Barry Rittman in August this year contributed to
> > histonet:
> > "Fixatives used to be described as additive or non
> > additive.
> > Additive fixatives are those in which the fixing
> > attaches to the
> > protein molecule.
> > Non additive fixatives on the other hand cause
> > in the proteins
> > that cause them to become changed, often used to
> > described as
> > denaturation.
> > e.g. formalin., picric acid are additive, ethanol
> > non additive.
> > Because of the complexity of fixation and the
> > variety of agents
> > used, the terms additive and non additive are not
> > commonly used."
> > The question I have been pondering is "What is the
> > preferred classification for fixatives that we
> > use?"
> > Luna suggested the terms hard and soft fixatives,
> > basing his classification on nuclear appearance.
> > What does everyone else think?
> > Regards and Happy Christmas and a good new year
> > Tony
> > =====
> > Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
> > Laboratory Manager
> > Histopathology Department
> > The Childrens Hospital at Westmead
> > Locked Bag 4001
> > WESTMEAD, 2145
> > AUSTRALIA
> > http://shopping.yahoo.com.au - Yahoo! Shopping
> > - Free CDs for thousands of Priority Shoppers!
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
The Childrens Hospital at Westmead
Locked Bag 4001
http://shopping.yahoo.com.au - Yahoo! Shopping
- Free CDs for thousands of Priority Shoppers!
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