RE: Fixation Classification

From:"Morken, Tim"

Tony, I think it would be something like "crosslinking" and
"non-crosslinking" which would take into account the actual mechanisms of,
say, formalin vs picric acid.

Tim Morken
CDC, Atlanta

-----Original Message-----
From: Tony Henwood []
Sent: Thursday, December 13, 2001 5:18 PM
To: Histonet
Subject: Fixation Classification

Hi all,
Barry Rittman in August this year contributed to

"Fixatives used to be described as additive or non
Additive fixatives are those in which the fixing agent
 attaches to the
protein molecule.
Non additive fixatives on the other hand cause changes
in the proteins
that cause them to become changed, often used to be
described as
e.g. formalin., picric acid are  additive, ethanol is
non additive.
Because of the complexity of fixation and the great
variety of agents
used, the terms additive and non additive are not now
commonly used."

The question I have been pondering is "What is the
preferred classification for fixatives that we should
Luna suggested the terms hard and soft fixatives,
basing his classification on nuclear appearance.
What does everyone else think?

Regards and Happy Christmas and a good new year


Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
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